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Dissolved Oxygen Probe AT-SB-PROBE-DO-P-C1

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Datasheet

1. Introduction

SENSBLUE RS-485 Dissolved Oxygen Probe AT-SB-PROBEDO-P is an integrated online fluorescence dissolved oxygen sensor designed based on the quenching principle of excited fluorescence by specific substances in physics. When the excitation light shines on the fluorescent substance on the surface of the fluorescent membrane head, the substance becomes excited and emits fluorescence. The extinguishing time of this fluorescence is affected by the concentration of oxygen molecules on the surface of the fluorescent membrane head. The oxygen concentration can be calculated by detecting the phase difference between the fluorescence and the excitation light and comparing it with the internal calibration curve. The final value is then output after temperature and salinity compensation.



2. Features

  • No electrolyte is required and it will not be polarized.
  • No oxygen consumption; not affected by flow rate.
  • Built‑in temperature sensor, automatic temperature compensation.
  • Built‑in salinity compensation, flexible parameter settings.
  • Free from interference by chemicals such as sulfides.
  • Small drift, rapid reaction, for more accurate measurement.
  • Long service cycle, lower cost of use.
  • Simple replacement of fluorescent cap.RS‑485 interface, Modbus/RTU protocol.
  • Low power consumption and anti‑interference


3. Technical Specifications

Model Pro Ultra
Case material POM, ABS/PC alloy, 316L stainless steel 316L Titanium Alloy
Measurement principle Fluorescence method
Range 0~20.00 mg/L (0~200% saturation, 25℃)
Resolution 0.01mg/L, 0.1℃
Accuracy ±2%, ±0.3℃ ±3%
Response time (T90) <30s <60s
Minimum detection limit 0.08mg/L
Calibration method Two-point calibration
Temperature compensation Automatic temperature compensation (Pt1000)
Output method RS-485 (Modbus RTU)
Storage temperature -5~65℃
Working conditions 0~50℃, ≤0.2MPa
Installation method Submersible installation, 3/4 NPT Max Depth 30m
Power consumption 10.1W@12V
Power supply 12~24V DC
Protection level IP68
Life of fluorescent membrane head 1 year (under normal use)
Cable length 10 meters, other lengths can be customized
Max water depth 30m


4. Dimensions and Wiring



Pinouts
1 - Pin 12-24V DC 4 - RS485 B-
2 - GND 5 – NA
3 - RS485 A+ 6 - NA
7 - NA

5. Installation

Install

The sensor needs to be installed below the liquid level. The installation and use should avoid any collisions or scraping against the surface of the fluorescent film head. The fluorescent film head should also be kept from contacting the bottom sediment. Remove the rubber cover when in use.

6. Maintenance

6.1 Use and maintenance

a) Maintenance schedule

Different from dissolved‑oxygen probe technology based on the electrochemical principle, the fluorescence dissolved‑oxygen probe does not consume oxygen and does not need to be cleaned frequently (except when used in viscous liquids).

Maintenance Task Recommended Maintenance Frequency
Cleaning sensor Wash every 30 days
Check for damage to sensors and caps Check every 30 days
Replace the fluorescent cap Replace it once a year
Calibrate (if required by the competent authority) According to the maintenance schedule required by the competent department

Note: The maintenance frequency in the above table is only recommended, and the maintenance personnel shall clean the sensor according to the actual use of the sensor, however, the replacement frequency of the fluorescent cap is recommended once a year.

6.2 Maintenance method

a) Sensor outer surface:

Clean the outer surface of the sensor with tap water, if there is still debris residue, wipe with wet soft cloth, for stubborn dirt, you can add household washing liquid to clean.

b) Surface of fluorescent film head:

If there is dirt on the surface of the fluorescent film head, please rinse it with clean water or wipe it gently with a soft cloth. Pay attention to the cleaning intensity to avoid causing scratches in the measuring area and affecting measurement accuracy.

c) Inside the fluorescent film head:

  It is generally unnecessary to clean. If water vapor or dust enters the fluorescent film head, the cleaning steps are as follows:

•Screw down the fluorescent film head;

•Rinse the inner surface of the fluorescent film head and the optical window of the sensor.

•For oily dirt, you can use household cleaning solution;

•Gently dry the water with a clean cloth and let dry;

Reinstall the fluorescent film head.

d) Check the cables of the sensor:

  There should be no damage to the skin and root of the cables;

Terminals should not be submerged in water; When the sensor is normally installed, the cable should not be tensioned, otherwise the internal wire of the cable will easily break, and the sensor cannot work normally.

e) Check whether the casing of the sensor is damaged by corrosion or other causes.

f) Daily storage of fluorescent film head:

  When not in use, a rubber protective cover with a wet sponge should be placed over the fluorescent film head to keep the surface of the measuring area wet. If the surface of the measuring area of the fluorescent film head becomes chronically dry, measurement errors or data instability may occur, and it needs to be soaked in water for 48 hours before use.

6.3 Frequently asked questions

Wrong Probable Cause Solution
The operating interface cannot connect or does not display the measurement results Error connecting controller to cable Reconnect the controller and cable
Cable failure Please contact us.
The fluorescent cap is not tightened or damaged Refit and tighten the fluorescent cap or replace the fluorescent cap.
The measured value is too high, too low, or the numerical value The outer surface of the fluorescent cap is attached to the outer object Clean the outer surface of the fluorescent

film head and agitate the film head during measurement.

The fluorescent cap was damaged
remains unstable. The fluorescent cap has exceeded

its service life

Replace the fluorescent cap
Temperature measurements change slowly. The temperature measuring area

(stainless steel housing) is attached to the exterior

Gently brush the attachment with a soft brush

6.4 Calibration of sensors

a) Zero Calibration

  Weigh 5 g of sodium sulphite using a balance, add 95 mL of water into a 250‑mL measuring cylinder, pour the water into a beaker, add the previously weighed sodium sulphite, and stir with a glass rod until fully dissolved to obtain a 5% sodium sulphite solution. Place the sensor in the solution and perform the zero‑point calibration after the reading has stabilized for three minutes. Refer to the Appendix for instructions.

Slope Calibration

  The sensor probe is placed in air‑saturated water, and the slope is calibrated after 3 minutes of numerical stability. The instructions refer to the appendix.

Preparation of Air Saturated Water:

  Add 2/3 volume of fresh distilled water to the constant‑temperature water bath to allow the porous plastic sheet to float on the water surface (see figure below). At the same time, use the bubbler (air pump) to aerate the water continuously for more than 1 hour. Then stop aeration and obtain air‑saturated water after approximately 20 minutes. Place the sensor into the water and calibrate the slope once the reading has stabilized.



Note: As an alternative, slope calibration can also be performed in water‑saturated air. Pour pure water into the calibration cup, keeping the liquid level column height slightly below 1 mm. After the sensor’s fluorescent membrane head is dipped in water, shake it lightly (to ensure that the surface of the fluorescent membrane is moist and there are no water droplets), and place the sensor vertically on the calibration cup column (as shown in the figure below, ensuring that the water surface does not touch the fluorescent membrane). Wait for 3 minutes for the value to stabilize before calibrating the slope.

6.5 Precautions

  • Avoid exposing the inner surface of the fluorescent film head to sunlight
  • Please do not touch the fluorescent film with your hands
  • Avoid bubbles adhering to the surface of the fluorescent film during measurement and calibration
  • Avoid applying any mechanical stress (pressure, scratches, etc.) directly to the fluorescent film during use

7. Appendix data communication

7.1 Data Format

The default data format for Modbus communication is: 9600, n, 8, 1 (baud rate 9600bps, 1 start bit, 8 data bits, no parity, 1 stop bit).

7.2 Modbus Frame Format (xx represents a byte)

a) Read data instruction frame

05 03 xx  xx xx  xx xx  xx
Address FC Register start

address

Number of Registers CRC check code (low bytes in front)

b)  Read data response frame

05 03 xx xx  xx xx  xx
Address FC Number of Bytes Response data CRC check code (low bytes in front)

c)  Write data instruction frame

05 06 xx  xx xx  xx xx  xx
Address FC Register address Read-in data CRC check code (low bytes in front)

d)  Data response frame

05 06 xx  xx xx  xx xx  xx
Address FC Register address Read-in data CRC check code (low bytes in front)

7.3 Register Address

Register Address Name Instruction Number of Registers Access Method
40001 (0x0000) Measured value + temperature Four double-byte integers: measured value, measured decimal places, temperature value, and temperature decimal places. 4 (8 bytes) Read
40005 (0x0004) Dissolved oxygen saturation (0–200%) Two double-byte integers: saturation value and decimal places. 2 (4 bytes) Read
44097 (0x1000) Zero calibration Calibrated in anoxic water. Write value 0; read returns zero offset. 1 (2 bytes) Write / Read
44101 (0x1004) Slope calibration Calibrate in air-saturated water. Write value 0; read returns slope value × 1000. 1 (2 bytes) Write / Read
44113 (0x1010) Temperature correction In solution, write the actual temperature value ×10. Read returns the temperature calibration offset ×10. 1 (2 bytes) Write / Read
44129 (0x1020) Salinity compensation Read/write value is salinity (PSU) ×10 used for compensation. Default = 0 (no compensation). Range: 0–500 (0–50.0 PSU). 1 (2 bytes) Write / Read
48195 (0x2002) Sensor address Default value is 5. Range: 1–255. 1 (2 bytes) Write / Read
48196 (0x2003) Baud rate Default: 9600. 0 → 9600 1 → 19200 1 (2 bytes) Write / Read
48225 (0x2020) Reset sensor Restores calibration values to default. Write value 0. Sensor must be recalibrated after reset. 1 (2 bytes) Write

7.4 Command example

a)  Measurement command:

  Function: Get the dissolved oxygen value and temperature measured by the sensor; the unit of dissolved oxygen is mg/L, and the unit of temperature is ℃.

Request frame: 05 03 00 00 00 04 45 8D

Response frame: 05 03 08 01 02 00 02 00 B0 00 01 DB 0C

Reading example:

DO value temperature scale
01 02 00 02 00 B0 00 01

For example, the DO value 01 02 indicates the hexadecimal DO reading, and 0002 indicates the dissolved oxygen value with 2 decimal places, which is converted to a decimal value of 2.58. The temperature value 00B0 represents the hexadecimal temperature reading, and 0001 indicates the temperature value with 1 decimal place, converted to a decimal value of 17.6.

b.) Calibration instruction

Zero calibration

Function: set the calibration value of dissolved oxygen zero point of electrode; Request frame:05 06 10 00 00 00 8C 8E

Acknowledgement frame :05 06 10 00 00 00 8C 8E Slope calibration

Function: set the slope calibration value of dissolved oxygen for the electrode; here the slope value is calibrated in air saturated water。

Request frame:05 06 10 04 00 00 CD 4F

Response frame: 05 06 10 04 00 00 CD 4F

c) Set the device ID address:

Function: set the MODBUS device address of the electrode;

Change the device address 05 to 01, with the following example Request frame

:05 06 20 02 00 01 E3 8E

Response frame: 05 06 20 02 00 01 E3 8E

d) Salinity compensation command:

Function: Set the salinity compensation of the sensor;

Measure the water body with a salinity of 35.0 PSU and add salinity compensation.

The example is as follows:

Request frame: 05 06 10 20 01 5E 0D 2C

Response frame: 05 06 10 20 01 5E 0D 2C

7.5 Error response

If the sensor does not execute the upper‑computer command correctly, the following format of information is returned:

Definition Address FC CODE CRC check
data ADDR COM+80H xx CRC 16
Number of bytes 1 1 1 2

a) CODE:

01 – Functional code error

03 – Data error

b) COM:Received function code

8. Applicable Accessories

Name Requirements
PA000000076 SENSBLUE MONARCH Solar Panel 1 Probe
PA000000079 SENSBLUE MONARCH Solar Panel 2 Probes
PA000000080 SENSBLUE MONARCH Solar Panel 1 Probe External Antenna
PA000000074 SENSBLUE MONARCH Solar Panel 2 Probes External Antenna
PA000000081 SENSBLUE MONARCH Opaque Case 1 Probe
PA000000082 SENSBLUE MONARCH Opaque Case 2 Probes
PA000000083 SENSBLUE MONARCH Opaque Case 1 Probe External Antenna
PA000000084 SENSBLUE MONARCH Opaque Case 2 Probes External Antenna
PA000000020 SENSBLUE MONARCH RS485 Probe Splitter Box (1 input -> 2 outputs)

9. Similar Products

Name Requirements
PA000000085 SENSBLUE RS-485 Dissolved Oxygen Probe AT-SB-PROBE-DO-T-C1, w/ 15m cable w/connector
PA000000086 SENSBLUE RS-485 Low Conductivity Probe AT-SB-PROBE-LC-P-C1, w/ 15m cable w/connector
PA000000087 SENSBLUE RS-485 Medium Conductivity Probe AT-SB-PROBE-MC-P-C1, w/ 15m cable w/connector
PA000000088 SENSBLUE RS-485 ORP Probe AT-SB-PROBE-ORP-P-C1, w/ 15m cable w/connector
PA000000089 SENSBLUE RS-485   Salinity/Conductivity/TDS Probe AT-SB-PROBE-S-P-C1, w/ 15m cable w/connector
PA000000090 SENSBLUE RS-485 Ammonia Nitrogen (NH4-N) Sensor Probe AT-SB-PROBE-NH4-P-C1, w/ 15m cable w/connector
PA000000091 SENSBLUE RS-485 Dissolved CO2 Sensor Probe AT-SB-PROBE-CO2-P-5000-C1, w/ 15m cable w/connector

10. Applicable certifications and standards

EMC Directive 2014/30/EU.

  • EN IEC 61326-1: 2021
  • EN 55011: 2016+A2: 2021
  • EN IEC 61000-3-2: 2019+A1: 2021
  • EN 61000-3-3: 2013+A2: 2021